The in Vitro Rooting of Pear Rootstock (Pyrus Communis Pyraster)

Select Volume / Issue:

• Volume 3,Issue 6, Nov. 2016

Year:

2016

Type of Publication:

Article

Keywords:

Pear Rootstock, Prunus pyraster, Rooting, Micropropagation, Proliferation, MS, WPM medium

Authors:

Papakosta, Elektra

Journal:

IJRAS

Volume:

3

Number:

6

Pages:

320-323

Month:

November

ISSN:

2348-3997

Note:

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. CC BY-NC-SA 4.0 Creative Commons License: https://creativecommons.org/licenses/by-nc-sa/4.0/

Abstract:

The purpose of this study is micropropagation with in vitro techniques of autochthonous wild pear (variety pyraster), determining the micropropagation protocol and rooting in the culture medium. For the cultivation of the pear rootstock were sampled two culture mediums: WPM (Lloyd & McCown, 1980) and universal MS medium (Murashige & Skoog, 1962). The first results were obtained in the shoot proliferation phase with BAP concentrated (0.35mg/l) after three subcultures of apical bud explants inoculated in growth medium. In the proliferation phase the WPM medium resulted with a mean number of shoots per plant (4) and shoots length (2cm), while the MS medium with mean number of shoots (5.1) and mean length of the shoots (2.6cm). The best results in rooting phase with IBA concentration (0.5 mg/l) were obtained in MS medium: rooting percentage 70%, mean number of roots (4.4) and mean length of the roots (1.8cm).

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